Clostridium Difficile Toxin A/B Rapid Test

Description

Product information

Clostridium Difficile Toxin A/B Rapid Test is a lateral flow, immunochromatographic rapid test for the qualitative detection of Clostridium difficile Toxin A and Toxin B in human feces.

Advantages of Clostridium difficile Toxin A/B test

• Easy to perform
• No complex sample collection needed
• Accurate test result

General information

Clostridium difficile is an anaerobic bacteria acting as an opportunistic pathogen: it grows in the intestine when the normal flora has been altered by treatment with antibiotics. Toxinogenic strains of Clostridium difficile cause infections from mild-diarrhea to pseudomembranous colitis, potentially leading to death. Disease is caused by two toxins produced by toxinogenic strains of C.difficile: Toxin A (tissue-damaging enterotoxin) and Toxin B (cytotoxin).

Some strains produce both toxins A and B, some others produce Toxin B only. The potential role of a third (binary) toxin in pathogenicity is still debated. Clostridium difficileToxinA+Toxin B Combo Rapid Test Cassette(Feces) detects two distinct antigens in fecal specimens for C. difficile, viz., Toxin A and Toxin B on two different test strips in a single test cassette, thus simultaneously detecting two antigens specific to Clostridium difficile.

For C.difficile-specific Toxin A

Testing The membrane is precoated with anti-C.diff Toxin A antibody and anti-C.diff Toxin A antibody on the test line region. During testing, the specimen reacts with the particle coated with anti-C.diff Toxin A antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-C.diff Toxin A antibody on the membrane and generate a colored line.

The presence of this colored line in the test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

For C.difficile-specifc Toxin B

Testing The membrane is precoated with anti-C.diff Toxin B antibody and anti-C.diff Toxin B antibody on the test line region. During testing, the specimen reacts with the particle coated with anti-C.diff Toxin B antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-C.diff Toxin B antibody on the membrane and generate a colored line.

The presence of this colored line in the test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.