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Myoglobin Rapid Test
The myoglobin test is a blood test that measures the levels of myoglobin, a muscle protein, in the bloodstream. This test is used to diagnose muscle damage, particularly in the context of heart attacks or muscle injuries. Elevated myoglobin levels can indicate muscle injury or heart muscle damage, helping healthcare professionals with diagnosis and treatment decisions.
Product information
Myoglobin test is a rapid qualitative assay for the detection of human myoglobin in serum, plasma or whole blood.
Advantages of Myoglobin test
- Easy to perform
- No complex sample collection needed
- Accurate test result
General information
Myoglobin is an intra cellular heme protein involved in the storage and transfer of oxygen to muscle tissues. In man myoglobin is one of the major proteins found in the myocardium and therefore it is not surprising that injury to cardiac muscle results in the release of myoglobin into the bloodstream.
Normal levels in human serum are about 60 ng/mL with elevations as high as 1,500 ng/mL seen in patients with acute myocardial infarction (AMI). Consequently, myoglobin measurements are valuable indications in the early diagnosis of AMI as myoglobin has been reported to rise within 4 hours after the onset of symptoms.
Myoglobin test is a rapid qualitative assay for the detection of human myoglobin in serum, plasma or whole blood. The method employs a unique combination of monoclonal-dye conjugate and polyclonal solid phase antibodies to identify myoglobin in the test samples with a high degree of specificity. As the sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the myoglobin forming an antibody antigen complex.
This complex binds to the anti-myoglobin antibody in the test zone and produces a pink-rose colour band when myoglobin concentration is higher than 100 ng/mL. In absence of myoglobin, there is no line in the test zone.
The reaction mixture continues flowing through the absorbent device past the positive reactive zone and control zone. Unbound conjugate binds to the reagents in the control zone, producing a pink-rose colour band, demonstrating that the reagents are functioning correctly.
Test procedure
- Allow specimen and Myoglobin Rapid Test to come to room temperature prior to testing.
- Remove the reaction device from its protective wrapper by tearing along the split.
- Label device with the patient ‘s name or control number.
- Fill the serum dropper with specimen (serum or plasma) and by holding it vertically, dispense one drop (25 μL) into sample well. If whole blood is used, dispense 2 drops (50 μL) into the well and wait for the whole blood sample to be completely absorbed before adding diluent.
- Add exactly 5 or 6 full drops of diluent (200 μL) in the sample well.
- Read the results after 5 to 10 minutes.
Interpretation of the results
Negative result: One colored band appears in the control zone. No apparent band in the test zone.
Positive result: In addition to the control band a clearly distinguishable band also appears on the test zone.
Invalid result: If there is no distinct colored band visible the control zone, the test is inconclusive for the corresponding parameter. In this case, it is recommended that the test be repeated.
Product name | Myoglobin Rapid Test |
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Detection | Myoglobin |
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