H. Pylori Antigen Rapid Test

Product information

H. Pylori Antigen test is a qualitative assay for the detection of H. pylori in stool samples. The method employs a unique combination of anti-human H. Pylori antibodies to specifically detect H. pylori with a high degree of sensitivity.

Advantages of H. Pylori Ag test

• Easy to perform
• No complex sample collection needed
• Accurate test result

General information

Helicobacter pylori (originally named Campylobacter pylori), when it was first discovered, is a spiral shaped microaerophilic bacillus which colonizes the surface epithelium of the gastric crypts and proximal duodenum. H .pylori is associated with a variety of gastro-intestinal diseases including chronic active-gastritis, gastric and duodenal ulcers and gastric adenocarcinoma. About 90% of patients with duodenal or gastric ulcers (demonstrated by culture and histological methods) are infected with H. pylori.

H. pylori infection can be detected either by invasive methods (biopsies culture, urease test) or by noninvasive methods (serology urea breath test (UBT) and faces test). UBT and faces test are the recommended methods for the follow up of patients after specific H. pylori eradication treatment. H. Pylori Antigen rapid test is a qualitative assay for the detection of H. pylori in stool samples.

The method employs a unique combination of anti-human H. pylori antibodies to specifically detect H. pylori with a high degree of sensitivity. As the samples flows through the absorbent device, the antihuman H. pylori antibodies dye conjugate binds to the H. pylori antigen, forming an antibody-antigen complex.

This complex binds to the H. pylori antibodies in the reaction zone (T) and produces a pink-rose color band. In the absence of H. pylori, there is no line in the reaction zone (T). The mixture continues flowing through the solid phase. Unbound conjugate binds to the reagents in the control zone (C) producing a pink-rose color band, demonstrating that the reagents are functioning correctly.

Test procedure

1. Allow the samples and reagents to come to room temperature prior to testing.
2. Remove the test device from the pouch.
3. Break the tip of the collection device, squeeze 5 drops (150 μL) of extracted sample into the sample well (▷) of the reaction device.
4. Read the results of the test 10 minutes after adding sample on the device. Do not interpret after 15 minutes.

Interpretation of the results

Negative result: One colored band appears in the control zone (C). No apparent band in the test zone (T). The sample does not contain Helicobacter pylori.

Positive result: In addition to the control band a clearly distinguishable band also appears in the test zone (T). The sample contains Helicobacter pylori.

Invalid result: If there is no distinct color band visible in the control zone (C), the test is inconclusive. It is recommended in this case to repeat the test.