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Mononucleosis Rapid Test
Mononucleosis, known as the “mono spot” test or the infectious mononucleosis test, is a diagnostic test that helps identify the presence of the Epstein-Barr virus (EBV) in a person’s blood. EBV is the virus responsible for causing mononucleosis, a common viral infection often referred to as the “kissing disease.” The test is used to confirm a mononucleosis diagnosis. It plays a crucial role in identifying the underlying cause of symptoms like fatigue, fever, and sore throat, enabling appropriate medical care and management.
Product information
The Mononucleosis Rapid Test is a rapid qualitative assay for the detection of human heterophile IM antibodies (IgM class) in serum, plasma or whole blood.
Advantages of Mononucleosis test
- Easy to perform
- No complex sample collection needed
- Accurate test result
General information
Infectious Mononucleosis (IM) is an acute disease caused by the Epstein-Barr virus (EBV). The most common symptoms are fatigue, pharyngitis, fever, lymphadenopathy, splenomegaly and hepatitis. Heterophile antibodies are the primary antibodies which appear in the patient’s serum, usually one to three weeks after the onset of symptoms and in 85% to 95% of the cases. These antibodies are mainly of IgM class and may be detected in patient serum over one year after the onset of illness. Reliable laboratory diagnosis of IM has been performed for over fifty years based on the detection of heterophile antibodies. These antibodies are directed against antigens found in bovine, sheep and horse erythrocytes.
The Mononucleosis Rapid Test is a rapid qualitative assay for the detection of human heterophile IM antibodies (IgM class) in serum, plasma or whole blood. The method employs a unique combination of anti IgM dye conjugate and horse erythrocytes extract fixed on the membrane to specifically detect IM heterophile antibodies with a high degree of sensitivity. As the sample flows through the absorbent device, the anti Ig M-dye conjugate binds to the IgM heterophile antibodies forming an antibody-antigen complex. This complex binds to the horse extract in the positive reaction zone (T) and produces a pink-rose color band.
In the absence of IgM heterophile antibodies, there is no line in the positive reaction zone (T). The mixture continues flowing through the absorbent device past the reaction zone and control zone (C). Unbound conjugate binds to the reagents in the control zone (C) producing a pink-rose color band, demonstrating that the reagents are functioning correctly.
Test procedure
- Remove the test device from the pouch.
- Add 25 μL of the positive control into the sample well on reaction device (▷).
- Using the dropper bottle, add 4 drops of diluent into the sample well (▷).
- Read the results of the test after 5 to 10 minutes.
Interpretation of the results
Negative result: Only one colored band appears on the control zone (C).
Positive result: In addition to the control band, a distinguishable band also appears in the test zone (T) indicating a positive result.
Invalid result: If there is no band in the control zone (C), the test is inconclusive. In this case, it is recommended that the test be repeated or a fresh specimen obtained and tested 48 hours later.
| Product name | Mononucleosis Rapid Test |
|---|---|
| Detection | Infectious Mononucleosis in Whole blood, Serum or Plasma |
| Type | |
| Sample Type | |
| Pack Size | |
| Format | |
| Analyte Detection |
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