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CK-MB Rapid Test
The CK-MB test, or Creatine Kinase-MB test, is a commercially available diagnostic blood test used to measure the levels of a specific enzyme called creatine kinase-MB in the bloodstream. This test is primarily used to assess and diagnose heart muscle damage, such as during a heart attack.
Elevated CK-MB levels in the blood can indicate myocardial injury, as this enzyme is primarily found in the heart muscle. It is an important tool for healthcare professionals to help confirm or rule out acute coronary syndrome and other cardiac conditions.
Product information
CK-MB (Creatine Kinase (MB isoenzyme) test is a rapid qualitative assay for the detection of CK-MB in serum.
Advantages of CK-MB test
- Easy to perform
- No complex sample collection needed
- Accurate test result
General information
Creatine Kinase is a dimer occurring in various human tissues in three isoenzymic forms, depending on the particular combination of its non identical submits : BB (brain type), MM (skeletal type), and MB (hybrid type). Creatine Kinase-MB isoenzyme (CK-MB) is well-documented as maker for acute myocardial infarction (AMI). It is found in blood within 6 hours of myocardial necrosis and peaked in 13-15 hours with a typical range of 39-185 ng/mL. Elevated levels persist for 2-3 days after the onset of chest pain. The CK-MB check-1 is a rapid qualitative assay for the detection of CK-MB in serum. The method employs a unique combination of monoclonal-dye conjugate and polyclonal solid phase antibodies to identify CK-MB in the test samples with a high degree of specificity. As the test sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the CK-MB forming an antibody-antigen complex. This complex binds to the anti-CK-MB antibody in the reaction zone and produces a pink-rose color band when CK-MB concentration is higher than 7 ng/mL. In the absence of CK-MB, there is no line in the reaction zone. The mixture continues flowing through the absorbent device past the reactive zone and control zone. Unbound conjugates binds to the reagents in the control zone, producing a pink-rose color band, demonstrating that the reagents are functioning correctly.
Test procedure
- Allow specimen and Myoglobin Rapid Test to come to room temperature prior to testing.
- Remove the reaction device from its protective wrapper by tearing along the split.
- Label device with the patient ‘s name or control number.
- Fill the serum dropper with specimen (serum or plasma) and by holding it vertically, dispense one drop (25 μL) into sample well. If whole blood is used, dispense 2 drops (50 μL) into the well and wait for the whole blood sample to be completely absorbed before adding diluent.
- Add exactly 5 or 6 full drops of diluent (200 μL) in the sample well.
- Read the results after 5 to 10 minutes.
Interpretation of the results
Negative result: One colored band appears in the control zone. No apparent band in the test zone.
Positive result:In addition to the control band a clearly distinguishable band also appears on the test zone.
Invalid result: If there is no distinct colored band visible the control zone, the test is inconclusive for the corresponding parameter. In this case, it is recommended that the test be repeated.
Product name | CK-MB Rapid Test |
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Detection | Cardiac Marker CK-MB |
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Analyte Detection |
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