Calprotectin Rapid Test

Product information

The Calprotectin Rapid Test is a immunochromatographic rapid test for the detection of Calprotectin in feces samples

Advantages of Calprotectin test

• Easy to perform
• No complex sample collection needed
• Accurate test result

General information

Calprotectin is a 36.5 kDa protein belonging to the S100 group of proteins (1). It represents up to 60% of the soluble protein content in the neutrophile granulocytes cytosol (2). Due to its ability to sequester manganes and zinc, calprotectin has recognized bacteriostatic and mycostatic activity which is comparable to antibiotics. Elevated levels of faecal calprotectin indicate the migration of neurophils to the intestinal mucosa which occurs during intestinal inflammation. The main diseases that cause or increased excretion of faecal calprotectin are inflammatory bowel diseases, coeliac diseases,
infectious colitis, intestinal cystic fibrosis and colorectal cancer (3,4). As the protein is resistant to enzymatic degradation, it has become a marker of choice which could be easily measured in feces (5).

Calprotectin Rapid Test is a fast, simple and highly sensitive test for the rapid and reliable detection of calprotectin antigen in the feces. After feces collection in a tube containing extraction solution, a few drops of this extract are added to the sample well (▷) of the reaction device.

As the test sample flows through the absorbent device, the anti-calprotectin monoclonal antibody-gold conjugate binds to the calprotectin antigen (when present in the sample), forming an antibody antigen complex. This complex binds to another anti calprotectin monoclonal antibody in the positive reaction window, producing a rosepink colored band when the calprotectin concentration is higher than 300ng/mL. In the absence of calprotectin, there is no line in the positive reaction zone (T). The reaction mixture continues flowing through the absorbent device, past the positive reaction zone (T) and control zone (C). Unbound conjugate binds to the reagent in the control window (C), producing a rose-pink colored band demonstrating that the reagents are functioning correctly.

Test procedure

1. Allow samples and reagents to return to room temperature prior to testing
2. Remove the test device from the pouch.
3. Break the purple tip of the collection device, squeeze 5 drops (150 μL) of mixture into the sample well (▷) of the reaction device.
4. Read the results of the test 10 minutes after addition of the sample of the device.

Interpretation of the results

Negative result: Only 1 colored band appears on the control zone (C). The sample does not contain calprotectin or the concentration is lower than 300ng/mL.

Positive result: In addition of the control band a clearly distinguishable band also appears on the test one (T). The sample contains calprotectin at a concentration higher than 300ng/mL.

Invalid result: If there is no distinct color band visible in the control zone (C), the test is inconclusive. In his case it is recommended that the test be repeated.