Ferritin Rapid Test

Product information

The FRT (Ferritin) test is a rapid qualitative assay for the detection of human FRT in serum, plasma or whole blood.

Advantages of FRT test

• Easy to perform
• No complex sample collection needed
• Accurate test result

General information

Ferritin is the form in which higher animals store iron intracellularly. It is present in all cells, with the highest concentrations in the liver, spleen and bone marrow. Ferritin is a protein consisting of 24 polypeptide subunits (Mr 450,000) assembled in the shape of a hollow sphere with an outer diameter of 12-14 nm. Stored iron is held within this sphere.

Clinical trials have shown that serum ferritin determination may be used for the diagnosis of iron deficiency anemia . As iron deficiency develops, iron stores are depleted before anemia becomes apparent. Serum ferritin is useful in detecting iron store depletion as the concentration drops to very low values. The FRT-check-1 test is a rapid qualitative assay for the
detection of human FRT in serum, plasma or whole blood.

The method employs an unique combination of monoclonal-dye conjugate and polyclonal solid phase antibodies to identify FRT in the test samples with a high degree of specificity. As the test sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the FRT forming an antibody-antigen complex.

This complex binds to the FRT antibody in the positive reaction zone and produces a rose-pink colored band when FRT concentration is higher than 20 ng/mL. In the absence of FRT, there is no line in the positive reaction zone. The reaction mixture continues flowing through the absorbent device past the reactive zone and control zone.
Unbound conjugate binds to the reagents in the control zone, producing a rose-pink colored band, demonstrating that the reagents are functioning correctly.

Test procedure

1. Allow samples and FRT test devices to come to room temperature prior to testing.
2. Remove the “reaction device” from its protective wrapper by tearing along the split.
3. Label device with the patient’s name or control number.
4. Fill the serum dropper with specimens (serum or plasma) and by holding it vertically, dispense one drop (25 μL) into sample well. If the whole blood is used, dispense two drops (50 μL) into well and wait for the whole blood sample to be completely absorbed before adding diluent.
5. Add exactly 5 or 6 full drops of diluent (200 μL) in the sample well.
6. Read the results after 10 to 15 minutes.

Interpretation of the results

Negative result: One coloured band appears. The Ferritin concentration is lower than 20 ng/mL. The patient is suffering from anemia

Positive result: Two clearly distinguishable bands appear indicating a positive result. The Ferritin concentration is higher than 20 ng/mL. There is no anemia in that case.

Invalid result: If there is no distinct color band visible in the test and control windows, the test is inconclusive. It is recommended in this case to repeat the test.