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Rota-Adenovirus Rapid Test
The Rota and adenovirus test is a diagnostic test used to detect the presence of rotavirus and adenovirus in stool samples. Rotavirus and adenovirus are two common viruses that can cause gastrointestinal infections, particularly in children. This test helps healthcare providers identify the cause of symptoms like diarrhea and vomiting.
Product information
The Rota-Adenovirus Test a lateral flow, qualitative immunochromatographic rapid test for the detection of both rotavirus and/or adenovirus in feces as a tool to assess infectious status by medical healthcare professionals.
Advantages of Rota-Adenovirus test
- Easy to perform
- No complex sample collection needed
- Accurate test result
General information
Rotavirus and Adenovirus are both a major cause of infectious gastroenteritis and related syndromes in infants and children. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly, and immunocompromised patients. In temperate climates, Rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported. With hospitalized children suffering from acute enteric disease up to 50 % of the analyzed specimen were positive for Rotavirus. The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Adenoviruses have been recovered from virtually every organ system of humans and have been associated with many clinical syndromes including pharyngitis, pharyngo conjunctival fever, bronchitis pneumonia for the respiratory tract in addition to gastroenteritis. Severe eye diseases like Epidemic keratoconjunctivitis (EKC) or acute hemorrhagic cystitis are also caused by Adenovirus. Rotavirus or Adenovirus infections can be easily transferred as the virus breeds in the gut and is secreted with faces. Therefore, nosocomial infections with these viruses are dangerous, particularly in pediatric wards and neonatal nurseries, and their management is difficult.
The Rota-Adenovirus Test is a lateral flow, qualitative immunochromatographic rapid test for the detection of Rotavirus and Adenovirus in human faces. The test device consists of a plastic housing containing two different sticks for the detection of Rotavirus and Adenovirus. A colored anti Rotavirus or Adenovirus monoclonal antibody colloidal gold conjugate is placed at the left end of the membrane. After collection in a tube containing the extraction solution, the faces sample is dissolved and few drops of this extract are added into each well (▷) of the reaction device. As the test sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the Rotavirus and Adenovirus antigen (when present in the sample) forming an antibody antigen complex. This complex binds to the polyclonal anti-Rotavirus or Adenovirus antibody in the positive reaction window producing a rose-pink colored band. In absence of
Rotavirus or Adenovirus, there is no line in the positive reaction zone. The reaction mixture continues flowing through the absorbent device, past the positive reaction zone and control zone. Unbound conjugate binds to the reagent in the control window producing a rose-pink colored band demonstrating that the reagents are functioning correctly.
Test procedure
- Bring all reagents at room temperature.
- Remove the test device from the pouch by tearing along the split.
- Open the plastic tube containing the extracted sample.
- Using the plastic pipette, add 5 drops (150 μL) of the extracted solution into each sample well (↕).
- Read the results of the test 10 minutes after addition of the sample to the device.
Interpretation of the results
Negative result Rota and/or Adeno: The (C) control line for Rota and/or Adeno is present, but the (T) line does not appear.
Positive result Rota and/or Adeno: The (C) control line and the (T) line for Rota and/or Adeno is present.
Invalid result Rota and/or Adeno: The (C) control line for Rota and/or Adeno does not appear.
Product name | Rota-Adenovirus Rapid Test |
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Detection | Rotavirus and Adenovirus |
Type | |
Sample Type | |
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Analyte Detection |
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